The Mitte Museum was created in 2004 from the merger of the Museum Mitte von Berlin with the local history museums Tiergarten and Wedding, as a result of the merger of the former districts of Mitte, Tiergarten and Wedding. It is located in one of Berlin's oldest surviving school buildings, dating from 1866, and underwent extensive renovation from 2016 to 2019.

The permanent exhibition, which opened in 2021, explores the question of by whom and for whom the city is made and postulates: As an autonomous actor, the city helps shape people's actions. Several thematic rooms present well-known and lesser-known aspects from the history of the historic districts of Mitte, Wedding and Tiergarten and the present of today's Greater Mitte district.

The exhibition "Gewachsen auf Sand" ("Grown on Sand") tells of the interplay of forces that form(ed), dissolve(ed) and continually recreate(ed) the urban space of Berlin's Mitte. Examples from the planning, society and culture of the city over the last 250 years are also reflected in creative and critical confrontations in the visual arts and literature, which are also presented.

The Mitte Museum is an institution of the Mitte District Office of Berlin, Office for Further Education and Culture, Department of Art, Culture, and History, Division for Remembrance Culture and History.