Museum Treptow was founded in 1991 and is located on the second floor of the former City Hall of Johannisthal. The Neorenaissance building was erected in 1906 and refurbished in 2005/2006 according to the strict guidelines for historical monuments.

The permanent exhibition under the title "Aus 200 Jahren Treptower Geschichte" ("From 200 Years of Treptow History") is devoted to several themes that previously were examined in special exhibitions. For example, they explore the Johannisthal Flugplatz – Germany's first motorised airfield – and the life of the brave pilot Melli Beese. 

Other chapters examine the Berlin industrial exhibition of 1896, the development of cycling in Berlin, Treptow as a recreational destination, the history of the Teltow Canal and life in the Treptow-Neukölln border area.

The comprehensive archive is open to the public. The museum places value on the inclusion of daily experience and stories and enables thus civic participation in its work.