Gardens have always been places of recreation and spiritual edification. However, they are not only seen as a symbol of the lost paradise, but also reflect the various social conditions. In all epochs, gardens have served to represent their owners, whose names are often closely associated with the gardens. In contrast, the gardeners involved in their creation and maintenance are usually less well known to the public. It was their comprehensive knowledge in all areas of horticulture and garden art that enabled the idea of the garden, its artistic design and decoration to come closer to the dream of paradise.

The Glienicke Court Gardener Museum is therefore dedicated to the profession of the court gardener and the spectrum of his practical and theoretical activities. Documents and exhibits from the possession of the court gardeners illustrate the facets of horticultural work from drawing plans and measuring to flower culture, describe training paths and career opportunities and illustrate their elevated social status.

The "Gallery of Prussian Court Gardeners" provides an overview of biographies and works in a compilation of portraits and illustrations. In the garden hall of Glienicke Palace, vedute from the 18th and 19th centuries convey a vivid impression of the landscape during the court gardeners' lifetime.